Coding

Part:BBa_K4343119

Designed by: Xiaolu Sun   Group: iGEM22_NNU-China   (2022-10-09)


LEU

LEU gene is also a commonly used screening marker in Y. lipolytica. Only strains with LEU gene can grow in the medium without Leu. Since the Y. lipolytica strains we used were Leu-deficient and the plasmid (BBa_K4343069) we constructed contained LEU gene. Only yeast cells that had been successfully introduced into the target segment with the LEU gene were able to grow on Leu-free medium. After screening, yeast cells containing both the target gene and the LEU gene were obtained. It is worth noting that in the present experiment the LEU screening marker cannot be lost and can only be used for a single transformation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 667
    Illegal XhoI site found at 696
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1236
    Illegal SapI site found at 1078


[edit]
Categories
Parameters
None